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KMID : 0359319930330010119
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Korean Journal of Veterinary Research
1993 Volume.33 No. 1 p.119 ~ p.123
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Immunocytochemical identification of Mycobacterium bovis in tissues
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Abstract
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The present study was intended to use the avidin-biotin-peroxidase-antiperoxidase complex (ABPAP) method for the identification of Mycobacterium bovis in the tissue sections of infected cattle. Antibodies and linksera for ABPAP procedure used in incubated order were rabbit anti-Mycobacterium polyvalent antibodies, goat anti-rabbit IgG, rabbit peroxidase-antiperoxidase complex, biotinyl-horse anti-rabbit IgG, and avidin-biotin-peroxidase complex.
Where the bacterial antigen was localized by ABPAP, a dark brown deposit occurred in the cytoplasms of macrophages and Langerhans´ giant cells of the granulomatous lesions.
The method approved to be highly specific for the identification of the bacteria and allowed a precise localization of the bacterial antigen in infected cells.
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